Skip Navigation LinksHome > Collagenase
Collagenase


Collagenases obtained from Clostridium histolyticum are enzymes that break the peptide bond in collagen. Clostridium histolyticum secretes Collagenase Class I and II, Neutral Protease and Clostripain.
These four enzymes work together to dissociate the extracellular matrix for cell isolation from tissue for clinical and research applications.
Crescent Chemical Co., Inc. supplies NORDMARK Animal-free GMP, GMP, and NB Collagenase, Neutral Protease and Clostripain for cell isolation and passaging. Nordmark is the largest producer of collagenase worldwide.
Click Here For More Information on Collagenase
Click here for Collagenase Calculator

Collagenase AF-1 GMP Grade (The World's First)
The AF-1 GMP clinical grade Collagenase is completely animal free and is produced to eliminate the potential of animal derived pathogens.
Click here for Collagenase AF-1 GMP Grade Product Notes

Neutral Protease AF GMP Grade (The World's First)
The AF GMP clinical grade Neutral Protease is completely animal free and is produced to eliminate the potential of animal derived pathogens.
Click here for Neutral Protease AF GMP Grade Product Notes

Collagenase NB 1 GMP Grade
Recommended for clinical applications which have to meet GMP requirements like tissue engineering and transplantation into humans.
Click here for Collagenase NB 1 GMP Product Notes

Neutral Protease NB GMP Grade
Recommended for clinical applications which have to meet GMP requirements like tissue engineering and transplantation into humans.
Click here for Neutral Protease NB GMP Product Notes

Collagenase NB 1 Premium Grade
This highly purified collagenase grade in combination with Neutral Protease NB is used for isolation of pancreatic islets of Langerhans from different species.
Click here for Collagenase NB 1 Premium Grade Product Notes

Neutral Protease NB
Neutral Protease NB is used in combination with purified Collagenase NB 1 Premium Grade, Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range.
Click here for Neutral Protease Product Notes

Neutral Protease NB High Active Grade
Neutral Protease NB High Active Grade contains an increased proteolytic activity and is used in combination with highly purified Collagenase NB 1 Premium Grade, Collagenase NB 4 Standard Grade or Collagenase NB 4G Proved Grade.
Click here for Neutral Protease High Active Grade Product Notes

Collagenase NB 4 Standard Grade
Applicable to research tissue dissociation such as isolation of human ADSCs, stem cells, chondrocytes, fibroblasts, neuronal, endothelial hepatocytes, cardiomyocytes, tumor, muscle, epithelial, gangliocytes, oocytes and periodontal cells.
Click here for Collagenase NB 4 Standard Grade Product Notes

Collagenase NB 4G Proved Grade
Suitable for isolation of a broad variety of cell types including adipocytes, fibroblasts, hepatocytes, chondrocytes, oocytes and stem cells.
Click here for Collagenase NB 4G Proved Grade Product Notes

Collagenase NB 5 Sterile Grade
Applicable to tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and rodent osteoblasts.
Click here for Collagenase NB 5 Sterile Grade Product Notes

Collagenase NB 6 GMP Grade
Applicable to clinical tissue dissociation such as isolation of human ADSCs, stem cells, chondrocytes, fibroblasts, neuronal, endothelial hepatocytes, cardiomyocytes, tumor, muscle, epithelial, gangliocytes, oocytes and periodontal cells.
Click here for Collagenase NB 6 GMP Product Notes

Collagenase NB 8 Broad Range
Increased collagenase activity. Applicable to tissue dissociation such as isolation of cardiomyocytes, porcine hepatocytes, rodent pancreatic tumors and thyroid cells.
Click here for Collagenase NB 8 Broad Range Grade Product Notes

Clostripain NB
Used in combination with other Collagenases and Neutral Proteases to improve your isolation results from human and animal tissue.
Click here for Clostripain NB Product Notes

Collagenase Properties and Applications, Collagenase Calculator, Drug Master File (DMF) and Frequently asked questions (FAQ):


Collagenase NB 1 Premium Grade
Cat. No. 17455.03
Pack Size ≥ 2,000 PZ U/Vial
  • Chromatographically highly purified
  • Class l and ll collagenases
  • Use in combination with NB Neutral Protease
  • PZ activity * ≥ 3.0 U/mg lyophilisate
  • Largely free of clostripain, trypsin-like proteases
  • Endotoxins ≥ 10 EU/mg lyophilisate
  • Collagen characterization
  • Isolation of pancreatic islets
  • Use in combination with Neutral Protease NB
  • Collagen characterization

Collagenase NB 1 GMP Grade
Cat. No. 17542.01
Pack Size ≥ 2,000 PZ U/Vial
  • Enzymatic properties see NB 1 Premium Grade
  • Manufactured according to Intl. cGMP guidelines
  • Isolation of pancreatic islets for transplantation into humans
  • Use in combination with Neutral Protease NB GMP Grade

Collagenase AF-1 GMP Grade
Cat. No. 17457-01
Pack Size ≥ 2,000 PZ U/Vial
  • Chromatographically highly purified
  • Class l and ll collagenases
  • Very low levels of endotoxins
  • Isolation of pancreatic islets sensitive liver, dental pulp cells

Collagenase NB 4 Standard Grade
Cat. No. 17454.01/ 1 g
Cat. No. 17454.02/ 500 mg
Cat. No. 17454.03/ 5 g
  • Class l and ll collagenases and a balanced ratio of proteolytic activities
  • PZ activity* ≥ 0.1 U/mg lyophilisate
  • Isolation of various cell types including neuronal and endothelial cells, chondrocytes, adipocytes epithelial cells, stem cells, gangliocytes and periodontal cells

Collagenase NB 4G Proved Grade
Cat. No. 17465.01/ 500 mg
Cat. No. 17465.02// 1 g
Cat. No. 17465.03/ 5 g
  • Class l and ll collagenases as well as a balanced mix of neutral protease, clostripain, and tryptic activities
  • PZ act.* ≥ 0.18 U/mg lyophilisate
  • Fuction tested for cell isolation
  • Isolation of adipocytes, fibroblasts hepatocytes, chondrocytes, stem cells, frog oocytes, mastocytes and tumor cells

Collagenase NB 5 Sterile Grade
Cat. No. 17459.03 / 1 g
  • Enzymatic properties see NB 4
  • Sterile according to European req. For medicinal prods. (Pharm. Eur.)
  • Application fields see NB 4
  • Sterile and ready to use

Collagenase NB 6 GMP Grade
Cat. No. 17458.03/ 1 g
Cat. No. 17458.04/ 100 mg
  • Enzymatic properties see NB 4
  • Aseptic production according to
  • European req. for medicinal prods.(Pharm. Eur.)
  • Manufactured according to Intl. cGMP guidelines
  • Application fields see NB 4
  • Production of cell as well as tissue engineering for transplantation into humans

Collagenase NB 8 Broad Range
Cat. No. 17456.01/ 250 mg
Cat. No. 17456.02/ 1 g
  • Class l and ll collagenases and reduced levels of clostripain, trypsin-like activities and neutral protease
  • PZ act.* ≥ 0.9 U/mg lyophilisate
  • Endotoxins ≥ 100 EU/mg lyophilisate
  • Isolation of cardiomyocytes, porcine hepatocytes, rodent pancreatic islets tumor and thyroid cells

Neutral Protease NB
Cat. No. 30301.11/ 50 U (DMC)
Cat. No. 30301.12/ 100 U (DMC)
  • Chromatographically purified neutral protease.
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB

Neutral Protease NB High Active Grade

Cat. No. 30302.02/ 80 U (DMC)
  • Chromatographically purified and contains an increased proteolytic activity
  • Largely free from collagenolytic activity (PZ U)
  • Endotoxin level is very low
  • Activity (DMC assay) ≥ 4.00 U/mg
  • Endotoxin ≥ 50.0 EU/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB 1 Premium Grade or another Collagenase NB product

Neutral Protease GMP Grade
Cat. No. 17452.01/ ≥ 2,000 U (PZ)
  • Chromatographically purified and largely free from collagenolytic activity (PZ U) and endotoxins
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB 1 GMP Grade or Collagenase NB 6 GMP Grade from various tissue types for clinical applications.

Neutral Protease AF GMP Grade
Cat No. 30306.01/ ≥ 2,000 U (PZ)
  • Chromatographically purified and largely free from collagenolytic activity (PZ U) as well as endotoxins
  • Absent of any animal-derived ingredients and thorough microbial analysis provide the highest possible safety
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase AF-1 GMP Grade from several tissue types for clinical applications

Collagenase N
Reference Content / ≥ 50 mg
Cat. No. 17463
  • Class l and ll collagenases and other proteolytic enzymes
  • Collagenase activity is regularly reevaluated
  • Reference substance for enzyme activity determination.

Collagenase N
Reference Identity / ≥ 50 mg
Cat. No. 17464
  • Class I and ll collagenases and other proteolytic enzymes
  • Reference substance for SDS-PAGE analysis, for incoming goods inspection according to cGMP

Anti-Collagenase
Cat. No. 58050 / 100 μ
  • Specifically against collagenases from C. histolyticum
  • lgG fraction purified by Protein G affinity chromatography
  • Use in ELISA and Western Blot experiments
  • Immunostaining of tissue
  • Analysis of residual collagenase impurities in cell preparations

Anti-Collagenase HRP
Cat. No. 58051 / 100 μ
  • Specifically against collagenases from C. histolyticum
  • lgG fraction purified by affinity chromatography
  • Conjugated with horseradish
  • Peroxidase
  • Use in ELISA and Western Blot experiments
  • Immunostaining of tissue
  • Analysis of residual collagenase impurities in cell preparations


* PZ activity acc. to Wϋnsch, measured at 25 ° C.
Drug Master File for Collagenase





Serva FAQ
Sort By
Show Per pages
Catalog # 17452.01 Size - >=2000U
Call For Price
Catalog # 17456.02 Size - 1G
Call For Price
Catalog # 17454.01 Size - 1G
Call For Price
Catalog # 17454.02 Size - 500MG
Call For Price
Catalog # 17454.03 Size - 5G
Call For Price
Catalog # 17456.01 Size - 250MG
Call For Price
Catalog # 17455.03 Size - >=2000U
Call For Price
Catalog # 17457.01 Size - >=2000U
Call For Price
Catalog # 17458.03 Size - 1G
Call For Price
Catalog # 17458.04 Size - 100MG
Call For Price
10 items of total 18
Top
Prev 1 2 Next