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Skip Navigation LinksHome > Collagenase Enzymes for Tissue Dissociation and Cell Isolation
Collagenase Enzymes for Tissue Dissociation and Cell Isolation

CRESCENT CHEMICAL CO., INC. is the distributor of NORDMARK COLLAGENASE NB, NEUTRAL PROTEASE NB and CLOSTRIPAIN NB enzymes in the Americas. Nordmark Collagenase, Neutral Protease and Clostripain enzymes are particularly well suited suited for various tissue dissociation and cell isolation for numerous clinical and research applications. The use of a cell type specific Collagenase NB, Neutral Protease NB ensures the isolation of viable cells with the highest yield. Nordmark is the world's largest collagenase producer and supplier. Nordmark Collagenase enzymes are manufactured under strict pharmaceutical regulations which guarantees stringent quality control, reliable lot-to-lot consistency and excellent product performance. TSE safety of the fermentation product has been certified by the European Authorities, EDQM and the supplier’s certificate of origins.

NORDMARK COLLAGENASE AF-1 GMP GRADE ENZYME FROM CLOSTRIDIUM HISTOLYTICUM WORLD'S FIRST ANIMAL FREE GMP GRADE COLLAGENASE Collagenase AF-1 GMP Grade is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This highly purified collagenase, often in combination with Neutral Protease AF GMP Grade (Cat. No. N0002936), is suitable for the dissociation of sensitive cells from several tissues, such as pancreas, liver and dental pulp. It has been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation.

NORDMARK NEUTRAL PROTEASE AF GMP GRADE ENZYME FROM CLOSTRIDIUM HISTOLYTICUM WORLD'S FIRST ANIMAL FREE GMP GRADE NEUTRAL PROTEASE Neutral Protease AF GMP Grade is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This purified metalloprotease is suitable for the dissociation of tissue in clinical applications. For especially gentle cell isolation it is recommended to use Neutral Protease AF GMP Grade in combination with highly purified Collagenase AF-1 GMP Grade (Cat. No. N0003554).

NORDMARK COLLAGENASE NB 1 GMP GRADE ENZYME FROM CLOSTRIDIUM HISTOLYTICUM NORDMARK GMP ENZYMES - HIGHEST QUALITY AND SAFETY FOR CLINICAL APPLICATIONS This highly purified collagenase quality is used for isolation of sensitive cells and guarantees high yields and viability. In combination with Neutral Protease NB GMP Grade (Cat. no. N0002936), Collagenase NB 1 GMP Grade is especially applicable to isolation of pancreatic islets of Langerhans. Collagenase NB 1 GMP Grade is recommended for applications which have to meet GMP requirements like tissue engineering and transplantation into humans.

NORDMARK NEUTRAL PROTEASE NB GMP GRADE ENZYME FROM CLOSTRIDIUM HISTOLYTICUM NORDMARK GMP ENZYMES - HIGHEST QUALITY AND SAFETY FOR CLINICAL APPLICATIONS Metalloprotease suitable for tissue dissociation applications. For cell isolation Neutral Protease NB GMP Grade is used in combination with purified Collagenase NB 1 GMP Grade (Cat. no. N0002937) or Collagenase NB 6 GMP Grade (Cat. no. N0002779) for applications which have to meet GMP requirements like tissue engineering and transplantation into humans.

COLLAGENASE NB 6 GMP GRADE ENZYME FROM CLOSTRIDIUM HISTOLYTICUM NORDMARK GMP ENZYMES - HIGHEST QUALITY AND SAFETY FOR CLINICAL APPLICATIONS For applications which have to meet GMP requirements like tissue engineering and transplantation. Collagenase NB 6 GMP Grade is applicable to tissue dissociation such as isolation of human ADSCs, stem cells, chondrocytes, fibroblasts, neuronal and endothelial cells.

NORDMARK NB 1 PREMIUM GRADE ENZYME FROM CLOSTRIDIUM HISTOLYTICUM NORDMARK COLLAGENASE NB - FIRST CHOICE FOR HIGH YIELD AND VIABLE CELLS This highly purified collagenase grade is used for isolation of sensitive cells and guarantees high yields and viability. In combination with Neutral Protease NB (Cat. no. S3030112) Collagenase NB 1 Premium Grade is especially applicable to isolation of pancreatic islets of Langerhans from different species like human or pig. For research use only. If cells are intended for transplantation into humans, Collagenase NB 1 GMP Grade (Cat. no. N0002937) is recommended.

NORDMARK NEUTRAL PROTEASE NB ENZYME FROM CLOSTRIDIUM HISTOLYTICUM NORDMARK COLLAGENASE NB - FIRST CHOICE FOR HIGH YIELD AND VIABLE CELLS Metalloprotease suitable for tissue dissociation applications. For cell isolation Neutral Protease NB is used in combination with purified Collagenase NB 1 Premium Grade (Cat. no. S1745503) as well as Collagenase NB 4 Standard Grade (Cat. no. S1745401) or Collagenase NB 8 Broad Range (Cat. no. S1745601). For research use only. If cells are intended for transplantation into humans, Neutral Protease NB GMP Grade (Cat. no. N0002936) is recommended.

NORDMARK NEUTRAL PROTEASE NB HIGH ACTIVE GRADE ENZYME FROM CLOSTRIDIUM HISTOLYTICUM NORDMARK COLLAGENASE NB - FIRST CHOICE FOR HIGH YIELD AND VIABLE CELLS Metalloprotease suitable for tissue dissociation applications. For cell isolation Neutral Protease NB High Active Grade is used in combination with highly purified Collagenase NB 1 Premium Grade (Cat. no. S1745503), Collagenase NB 4 Standard Grade (Cat. no. S1745401) or Collagenase NB 4G Proved Grade (Cat. no. S1746501).

NORDMARK NB 4 STANDARD GRADE ENZYME FROM CLOSTRIDIUM HISTOLYTICUM NORDMARK COLLAGENASE NB - FIRST CHOICE FOR HIGH YIELD AND VIABLE CELLS As Collagenase NB 4 Standard Grade has comparable enzymatic properties to Collagenase NB 5 Sterile Grade (Cat. no. N0002778) and Collagenase NB 6 GMP Grade (Cat. no.N0002779), this enzyme might also be used in order to establish applications which have to meet sterile or GMP requirements like tissue engineering and transplantation.

NORDMARK NB 4G PROVED GRADE ENZYME FROM CLOSTRIDIUM HISTOLYTICUM NORDMARK COLLAGENASE NB - FIRST CHOICE FOR HIGH YIELD AND VIABLE CELLS Suitable for isolation of a broad variety of cell types, especially for fibroblasts, human and rodent hepatocytes, frog oocytes and epithelial cells.

NORDMARK NB 5 STERILE GRADE ENZYME FROM CLOSTRIDIUM HISTOLYTICUM NORDMARK COLLAGENASE NB - FIRST CHOICE FOR HIGH YIELD AND VIABLE CELLS Collagenase NB 5 Sterile Grade is applicable to tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and rodent osteoblasts. See Collagenase NB 6 GMP Grade (Cat. no. N0002779) for clinical applications like tissue engineering and transplantation.

NORDMARK NB 8 BROAD RANGE ENZYME FROM CLOSTRIDIUM HISTOLYTICUM NORDMARK COLLAGENASE NB - FIRST CHOICE FOR HIGH YIELD AND VIABLE CELLS Suitable for a broad range of different applications in tissue dissociation such as isolation of rodent pancreatic islets of Langerhans, human cardiomyocytes, tumor cells, muscle cells, osteoblasts and porcine hepatocytes. Increased collagenase activity compared to Collagenase NB 4 Standard Grade (Cat. no. S1745401) and Collagenase NB 4G Proved Grade (Cat. no. S1746501).

NORDMARK CLOSTRIPAIN NB ENZYME FROM CLOSTRIDIUM HISTOLYTICUM CLOSTRIPAIN NB - FINE TUNE YOUR CELL ISOLATION Clostripain also known as Clostridiopeptidase B or Endoproteinase Arg-C is a proteinase that cleaves peptide bonds on the carboxyl side of arginine. This protein is a heterodimer composed of a heavy and a light chain that are held together via strong noncovalent forces. As a proteolytic enzyme it can be used for isolation of various cell types by tissue dissociation. Clostripain exists in two different forms, an oxidized, native condition and a reduced activated form that differ in enzymatic activity. The native clostripain can be activated with reducing agents (e.g. DTT, Cysteine) resulting in a higher activity of the enzyme. Clostripain NB is used in combination with purified Collagenase NB 1 Premium Grade (Cat. no. S1745503) and/or Neutral Protease NB (Cat. No. S3030112) as well as Collagenase NB 4 Standard Grade (Cat. no. S1745401) or Collagenase NB 8 Broad Range (Cat. no. S1745601). For research use only.

Collagenase NB, Neutral Protease NB, Collagenase N Reference Products and Anti-Collagenase Antibodies properties and applications include:


NB 1 Premium Grade
Cat. No. 17455.03
Pack Size ≥ 2,000 PZ U/Vial
  • Chromatographically highly purified
  • Class l and ll collagenases
  • Use in combination with NB Neutral Protease
  • PZ activity * ≥ 3.0 U/mg lyophilisate
  • Largely free of clostripain, trypsin-like proteases
  • Endotoxins ≥ 10 EU/mg lyophilisate
  • Collagen characterization
  • Isolation of pancreatic islets
  • Use in combination with Neutral Protease NB
  • Collagen characterization

NB 1 GMP Grade
Cat. No. 17542.01
Pack Size ≥ 2,000 PZ U/Vial
  • Enzymatic properties see NB 1 Premium Grade
  • Manufactured according to Intl. cGMP guidelines
  • Isolation of pancreatic islets for transplantation into humans
  • Use in combination with Neutral Protease NB GMP Grade

AF-1 GMP Grade
Cat. No. 17457-01
Pack Size ≥ 2,000 PZ U/Vial
  • Chromatographically highly purified
  • Class l and ll collagenases
  • Very low levels of endotoxins
  • Isolation of pancreatic islets sensitive liver, dental pulp cells

NB 4 Standard Grade
Cat. No. 17454.01/ 1 g
Cat. No. 17454.02/ 500 mg
Cat. No. 17454.03/ 5 g
  • Class l and ll collagenases and a balanced ratio of proteolytic activities
  • PZ activity* ≥ 0.1 U/mg lyophilisate
  • Isolation of various cell types including neuronal and endothelial cells, chondrocytes, adipocytes epithelial cells, stem cells, gangliocytes and periodontal cells

NB 4G Proved Grade
Cat. No. 17465.01/ 500 mg
Cat. No. 17465.02// 1 g
Cat. No. 17465.03/ 5 g
  • Class l and ll collagenases as well as a balanced mix of neutral protease, clostripain, and tryptic activities
  • PZ act.* ≥ 0.18 U/mg lyophilisate
  • Fuction tested for cell isolation
  • Isolation of adipocytes, fibroblasts hepatocytes, chondrocytes, stem cells, frog oocytes, mastocytes and tumor cells

NB 5 Sterile Grade
Cat. No. 17459.03 / 1 g
  • Enzymatic properties see NB 4
  • Sterile according to European req. For medicinal prods. (Pharm. Eur.)
  • Application fields see NB 4
  • Sterile and ready to use

NB 6 GMP Grade
Cat. No. 17458.03/ 1 g
Cat. No. 17458.04/ 100 mg
  • Enzymatic properties see NB 4
  • Aseptic production according to
  • European req. for medicinal prods.(Pharm. Eur.)
  • Manufactured according to Intl. cGMP guidelines
  • Application fields see NB 4
  • Production of cell as well as tissue engineering for transplantation into humans

NB 8 Broad Range
Cat. No. 17456.01/ 250 mg
Cat. No. 17456.02/ 1 g
  • Class l and ll collagenases and reduced levels of clostripain, trypsin-like activities and neutral protease
  • PZ act.* ≥ 0.9 U/mg lyophilisate
  • Endotoxins ≥ 100 EU/mg lyophilisate
  • Isolation of cardiomyocytes, porcine hepatocytes, rodent pancreatic islets tumor and thyroid cells

NB Neutral Protease
Cat. No. 30301.11/ 50 U (DMC)
Cat. No. 30301.12/ 100 U (DMC)
  • Chromatographically purified neutral protease.
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB

NB Neutral Protease
High Active Grade

Cat. No. 30302.02/ 80 U (DMC)
  • Chromatographically purified and contains an increased proteolytic activity
  • Largely free from collagenolytic activity (PZ U)
  • Endotoxin level is very low
  • Activity (DMC assay) ≥ 4.00 U/mg
  • Endotoxin ≥ 50.0 EU/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB 1 Premium Grade or another Collagenase NB product

NB Neutral Protease GMP Grade
Cat. No. 17452.01/ ≥ 2,000 U (PZ)
  • Chromatographically purified and largely free from collagenolytic activity (PZ U) and endotoxins
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB 1 GMP Grade or Collagenase NB 6 GMP Grade from various tissue types for clinical applications.

NB Neutral Protease AF GMP Grade
Cat No. 30306.01/ ≥ 2,000 U (PZ)
  • Chromatographically purified and largely free from collagenolytic activity (PZ U) as well as endotoxins
  • Absent of any animal-derived ingredients and thorough microbial analysis provide the highest possible safety
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase AF-1 GMP Grade from several tissue types for clinical applications

Collagenase N
Reference Content / ≥ 50 mg
Cat. No. 17463
  • Class l and ll collagenases and other proteolytic enzymes
  • Collagenase activity is regularly reevaluated
  • Reference substance for enzyme activity determination.

Collagenase N
Reference Identity / ≥ 50 mg
Cat. No. 17464
  • Class I and ll collagenases and other proteolytic enzymes
  • Reference substance for SDS-PAGE analysis, for incoming goods inspection according to cGMP

Anti-Collagenase
Cat. No. 58050 / 100 μ
  • Specifically against collagenases from C. histolyticum
  • lgG fraction purified by Protein G affinity chromatography
  • Use in ELISA and Western Blot experiments
  • Immunostaining of tissue
  • Analysis of residual collagenase impurities in cell preparations

Anti-Collagenase HRP
Cat. No. 58051 / 100 μ
  • Specifically against collagenases from C. histolyticum
  • lgG fraction purified by affinity chromatography
  • Conjugated with horseradish
  • Peroxidase
  • Use in ELISA and Western Blot experiments
  • Immunostaining of tissue
  • Analysis of residual collagenase impurities in cell preparations


* PZ activity acc. to Wϋnsch, measured at 25 ° C.
Drug Master File for Collagenase

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