Crescent Chemical Co., Inc. is the distributor of SERVA Collagenase NB and Neutral Protease NB in the US and Canada.  SERVA Collagenase NB are particularly well suited for various tissue dissociation and cell isolation applications.  They are manufactured by the pharmaceutical company Nordmark under strict pharmaceutical standards which guarantees stringent quality control, reliable lot-to-lot consistency and excellent product performance. TSE safety of the fermentation product has been certified by the European Authorities, EDQM and the supplier’s certificate of origins.

For further application information on your specific cell type or if you have any questions do not hesitate to contact us: collagenase@creschem.com

For more information click here

For the isolation of each cell type, a specific isolation procedure has to be applied to receive optimal yields of viable cells. The Crescent Calculator for SERVA Collagenase NB will help you to achieve the recommended enzymatic activity in your buffer.

The SERVA Collagenase & Neutral Protease Product line consists of the following products:

Material Safety Data Sheets are available for download on the bottom of this page.

• Collagenase NB 1 Premium Grade (item # 17455) & Neutral Protease NB (item # 30301)
  • Suitable for the isolation of pancreatic islets of Langerhans from different species like human or pig
  • Collagenase NB 1 - chromatographically highly purified enzyme containing collagenase class I and class II and very low levels of proteolytic activities and bacterial endotoxins
  • Used in the isolation of sensitive cells and guarantees high yields and viability
  • Neutral Protease - chromatographically purified essential additive for isolation of pancreatic islets
  • Provided separately allowing individual dosing and high stability of enzymes

• Collagenase NB 1 GMP Grade (item # 17452) & Neutral Protease GMP Grade (item # 30303)
  • Similar enzymatic properties to the Collagenase NB 1 Premium Grade and Neutral Protease NB
  • Suitable for applications that have to meet cGMP requirements like transplantation into humans
  • Several documents including TSE safety certificate and viral safety study are available for customers
  • For further Information regarding Pancreatic islet isolation for human transplantation click here

• Neutral Protease NB High Active Grade (item # 30302)
  • Higher activity and higher purity compared to Neutral Protease NB
  • Tested successfully for isolation of human islet from pancreas
  • Derived from Clostridium histolyticum

• Collagenase NB 4 Standard Grade (item # 17454)
  • Contains Collagenase class I and class II and a balanced mix of neutral protease, clostripain and trypsin-like activities 
  • Suitable for mild and effective isolation of a broad variety of cells including but not limited to adipocytes, adipose derived stem cells, hepatocytes, fibroblasts, chondrocytes, endothelial cells, neuronal cells and osteoblasts 

• Collagenase NB 5 Sterile Grade (item # 17459)
  • Similar properties to Collagenase NB4
  • Manufactured under aseptic conditions according to European Pharmacopoeia

• Collagenase NB 6 GMP Grade (item # 17458)
  • Similar enzymatic properties to the Collagenase NB 4 and NB 5
  • Suitable for applications that have to meet cGMP requirements like tissue engineering and transplantation into humans
  • Several documents including TSE safety certificate and viral safety study are available for customers
  • For further Information regarding Isolation and Passaging of Stem Cellsfor human transplantation click here

• Collagenase NB 8 Broad Range (item # 17456)
  • Characterized by increased collagenase activity and reduced levels of proteolytic activities and bacterial endotoxins.
  • Suitable for isolation of a broad variety of cells including but not limited to pancreatic islets, hepatocytes, muscle cells, cardiomyocytes and tumor cells.

• Collagenase NB 4G Proved Grade (item # 17465)

  • Characterized by increased collagenase activity compared to Collagenase NB4 and contains a mix of proteolytic activities
  • Suitable for isolation of epithelial cells and oocytes.

Anti-Frustration Kit