Crescent Chemical Co., Inc. supplies Nordmark Collagenase NB obtained from Clostridium histolyticum. Collagenases obtained from Clostridium histolyticum are enzymes that break the peptide bond in collagen. Clostridium histolyticum secretes Collagenase Class I and II, Neutral Protease and Clostripain. These four enzymes work together to dissociate the extracellular matrix for cell isolation from tissue for clinical and research applications. Scroll down for Crescent's line of Nordmark Collagenase NB and Product Notes. CLICK HERE FOR APPLICATION AND PROTOCOL INFORMATION
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New Nordmark Collagenase NB 7D Gentle Grade
Nordmark Collagenase AF-1 GMP Grade (The World's First Animal-free Collagenase)
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Nordmark Neutral Protease AF GMP Grade (The World's First Animal-free Neutral Protease)
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Nordmark Collagenase NB 1 GMP Grade
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Nordmark Neutral Protease NB GMP Grade
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Nordmark Collagenase NB 1 Premium Grade
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Nordmark Neutral Protease NB
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Nordmark Neutral Protease NB High Active Grade
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Nordmark Collagenase NB 4 Standard Grade
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Nordmark Collagenase NB 4G Proved Grade
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Nordmark Collagenase NB 5 Sterile Grade
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Nordmark Collagenase NB 6 GMP Grade
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Nordmark Collagenase NB 8 Broad Range
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Nordmark Clostripain NB Used in combination with other Collagenases and Neutral Proteases to improve your isolation results from human and animal tissue. Click here for Nordmark Clostripain NB Product Notes
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Collagenase N, Anti Collagenase, Collagenase Calculator, Drug Master File (DMF) and Frequently asked questions (FAQ): Collagenase N Reference Content / ≥ 50 mg Cat. No. 17463 Class l and ll collagenases and other proteolytic enzymes Collagenase activity is regularly reevaluated Reference substance for enzyme activity determination. Collagenase N Reference Identity / ≥ 50 mg Cat. No. 17464 Class I and ll collagenases and other proteolytic enzymes Reference substance for SDS-PAGE analysis, for incoming goods inspection according to cGMP Anti-Collagenase Cat. No. 58050 / 100 μ Specifically against collagenases from C. histolyticum lgG fraction purified by Protein G affinity chromatography Use in ELISA and Western Blot experiments Immunostaining of tissue Analysis of residual collagenase impurities in cell preparations Anti-Collagenase HRP Cat. No. 58051 / 100 μ Specifically against collagenases from C. histolyticum lgG fraction purified by affinity chromatography Conjugated with horseradish Peroxidase Use in ELISA and Western Blot experiments Immunostaining of tissue Analysis of residual collagenase impurities in cell preparations * PZ activity acc. to Wϋnsch, measured at 25 ° C. DRUG MASTER FILE FOR COLLAGENASE
DRUG MASTER FILE FOR COLLAGENASE
Nordmark Neutral Protease AF GMP Grade (The World's First)
Crescent Chemical Co., Inc. supplies Nordmark Clostripain NB. Clostripain (also known as Clostridiopeptidase B or Endoproteinase Arg-C) is a proteinase that cleaves peptide bonds on the carboxyl side of arginine. This protein is a heterodimer composed of a heavy and a light chain that are held together via strong noncovalent forces. As a proteolytic enzyme it can be used for isolation of various cell types by tissue dissociation. Clostripain exists in two different forms, an oxidized, native condition and a reduced activated form that differ in enzymatic activity. The native clostripain can be activated with reducing agents (e.g. DTT, Cysteine) resulting in a higher activity of the enzyme.
Click here for Nordmark Clostripain NB Product Notes Clostripain, the Missing Link in the Enzyme Blend for Efficient Human Islet Isolation 2017 - IPITA Islet Isolation Synergistic Effect of Neutral Protease and Clostripain on Rat Pancreatic Islet Isolation
Clostripain, the Missing Link in the Enzyme Blend for Efficient Human Islet Isolation 2017 - IPITA Islet Isolation Synergistic Effect of Neutral Protease and Clostripain on Rat Pancreatic Islet Isolation
Crescent Chemical Co., Inc. is the US distributor for SERVA Electrophoresis GmbH, a leading manufacturer of specialty reagents for SDS, Native PAGE electrophoresis and Isoelectric Focusing. SERVA is also a certified ISO 9001 supplier of biochemical products. All Serva products are subject to stringent quality control to meet the specifications stated in the Certificate of Analysis provided with each product.
Ultroser™ G serum substitute can successfully replace FCS (fetal calf serum) in cell culture medium. It is designed for the in vitro culture of anchorage-dependent cells. It is specially tested as the primary culture from amniotic liquid cells and is designed to confirm their ability for karyotyping.
Ultroser G serum substitute is CE mark-certified.
Ultroser G serum substitute has a concentration five times higher than fetal calf serum (2 % of reconstituted Ultroser G serum substitute is equivalent to 10% fetal calf serum in the basal medium).
Quality Control Tests Tests usually performed on Ultroser G serum substitute are:
Cells successfully tested with Ultroser™ G serum substitute:
Ultroser G serum substitute is available as a lyophilized powder. The product is shipped at ambient temperature and must be stored at 2-8 degrees C.