Crescent Chemical Co., Inc. supplies Nordmark Collagenase NB obtained from Clostridium histolyticum.
Collagenases obtained from Clostridium histolyticum are enzymes that break the peptide bond in collagen. Clostridium histolyticum secretes Collagenase Class I and II, Neutral Protease and Clostripain.
These four enzymes work together to dissociate the extracellular matrix for cell isolation from tissue for clinical and research applications.
Scroll down for Crescent's line of Nordmark Collagenase NB and Product Notes.

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New Nordmark Collagenase NB 7D Gentle Grade

• Catalog    #17474.01    500 mg
• Catalog    #17474.02    1 gm
• Catalog    #17474.03    5 gm
• Class I and II Collagenase and reduced trypsin like activities
• PZ activity    ≥ 0.2 U/mg Lyphosilate
• Trypsin like activity (BAEE) ≤ 0.2 U/mg Lyphosilate
• Ratio Collagenase Trypsin ≥ 3.0
• Used for a variety of applications where gentle conditions are beneficial.

Nordmark Collagenase AF-1 GMP Grade (The World's First Animal-free Collagenase)

• Cat. No. 17457.01
• ≥ 2,000 U(PZ)
• Completely Animal-free
• Chromatographically highly purified
• Class l and ll collagenases
• Very low levels of endotoxins
• PZ activity (Wünsch) ≥ 3.00 U/mg
• Isolation of especially sensitive cells including pancreatic islets, liver and dental pulp cells

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Nordmark Neutral Protease AF GMP Grade (The World's First Animal-free Neutral Protease)

• Cat No. 30306.01
• ≥ 100 U(DMC)
• Chromatographically purified and largely free from collagenolytic activity (PZ U) as well as endotoxins
• Absent of any animal-derived ingredients and thorough microbial analysis provide the highest possible safety
• Activity (DMC assay) ≥ 0.50 U/mg



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Nordmark Collagenase NB 1 GMP Grade


• Cat. 17452.01
• 2,000 U(PZ)
• Manufactured according to Intl. cGMP guidelines
• PZ activity (Wünsch) ≥ 3.00 U/mg
• Isolation of pancreatic islets for transplantation into humans
• Use in combination with Nordmark Neutral Protease NB GMP Grade



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Nordmark Neutral Protease NB GMP Grade


• Cat. No. 17452.01
• ≥ 100 U(DMC)
• Chromatographically purified and largely free from collagenolytic activity (PZ U) and endotoxins
• Activity (DMC assay) ≥ 0.50 U/mg
• Cell isolation in combination with Collagenase NB 1 GMP Grade or Collagenase NB 6 GMP Grade from various tissue types for clinical applications.



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Nordmark Collagenase NB 1 Premium Grade


• Cat. No. 17455.03
• ≥ 2,000 U(PZ)
• Chromatographically highly purified
• Class l and ll collagenases
• Use in combination with Nordmark Neutral Protease NB
• PZ activity * ≥ 3.0 U/mg lyophilisate
• Largely free of clostripain, trypsin-like proteases
• Endotoxins ≥ 10 EU/mg lyophilisate
• Collagen characterization
• Isolation of pancreatic islets



Click here for Nordmark Collagenase NB 1 Premium Grade Product Notes

Nordmark Neutral Protease NB


• Cat. No. 30301.11/ 50 U (DMC)
• Cat. No. 30301.12/ 100 U (DMC)
• Chromatographically purified neutral protease.
• Activity (DMC assay) ≥ 0.50 U/mg
• Cell isolation in combination with Nordmark Collagenase NB



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Nordmark Neutral Protease NB High Active Grade


• Cat. No. 30302.02
• 80 U(DMC)
• Chromatographically purified and contains an increased proteolytic activity
• Largely free from collagenolytic activity (PZ U)
• Endotoxin level is very low
• Activity (DMC assay) ≥ 4.00 U/mg
• Endotoxin ≥ 50.0 EU/mg
• Cell isolation in combination with Nordmark Collagenase NB product



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Nordmark Collagenase NB 4 Standard Grade


• Cat. No. 17454.01/ 1 g
• Cat. No. 17454.02/ 500 mg
• Cat. No. 17454.03/ 5 g
• Class l and ll collagenases and a balanced ratio of proteolytic activities
• PZ activity* ≥ 0.1 U/mg lyophilisate
• Isolation of various cell types including neuronal and endothelial cells, chondrocytes, adipocytes epithelial cells, stem cells, gangliocytes and periodontal cells



Click here for Nordmark Collagenase NB 4 Standard Grade Product Notes

Nordmark Collagenase NB 4G Proved Grade


• Cat. No. 17465.01/ 500 mg
• Cat. No. 17465.02// 1 g
• Cat. No. 17465.03/ 5 g
• Class l and ll collagenases as well as a balanced mix of neutral protease, clostripain, and tryptic activities
• PZ act.* ≥ 0.18 U/mg lyophilisate
• Fuction tested for cell isolation
• Isolation of adipocytes, fibroblasts hepatocytes, chondrocytes, stem cells, frog oocytes, mastocytes and tumor cells



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Nordmark Collagenase NB 5 Sterile Grade


• Cat. No. 17459.03 / 1 g
• Sterile according to European req. For medicinal prods. (Pharm. Eur.)
• PZ activity (Wünsch): ≥ 0.10 U/mg
• Applicable to tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and rodent osteoblasts.



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Nordmark Collagenase NB 6 GMP Grade


• Cat. No. 17458.03/ 1 g
• Cat. No. 17458.04/ 100 mg
• Aseptic production according to European req. for medicinal prods.(Pharm. Eur.)
• Manufactured according to Intl. cGMP guidelines
• PZ activity (Wünsch): ≥ 0.100 U/mg
• Applicable to clinical tissue dissociation such as isolation of human ADSCs, stem cells, chondrocytes, fibroblasts, neuronal, endothelial hepatocytes, cardiomyocytes, tumor, muscle, epithelial, gangliocytes, oocytes and periodontal cells.



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Nordmark Collagenase NB 8 Broad Range


• Cat. No. 17456.01/ 250 mg
• Cat. No. 17456.02/ 1 g
• Class l and ll collagenases and reduced levels of clostripain, trypsin-like activities and neutral protease
• PZ act.* ≥ 0.9 U/mg lyophilisate
• Endotoxins ≥ 100 EU/mg lyophilisate
• Isolation of cardiomyocytes, porcine hepatocytes, rodent pancreatic islets tumor and thyroid cells.



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Nordmark Clostripain NB
Used in combination with other Collagenases and Neutral Proteases to improve your isolation results from human and animal tissue.


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Collagenase N, Anti Collagenase, Collagenase Calculator, Drug Master File (DMF) and Frequently asked questions (FAQ):

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Collagenase N
Reference Content / ≥ 50 mg
Cat. No. 17463
• Class l and ll collagenases and other proteolytic enzymes
• Collagenase activity is regularly reevaluated
• Reference substance for enzyme activity determination.

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Collagenase N
Reference Identity / ≥ 50 mg
Cat. No. 17464
• Class I and ll collagenases and other proteolytic enzymes
• Reference substance for SDS-PAGE analysis, for incoming goods inspection according to cGMP

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Anti-Collagenase
Cat. No. 58050 / 100 μ
• Specifically against collagenases from C. histolyticum
• lgG fraction purified by Protein G affinity chromatography
• Use in ELISA and Western Blot experiments
• Immunostaining of tissue
• Analysis of residual collagenase impurities in cell preparations

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Anti-Collagenase HRP
Cat. No. 58051 / 100 μ
• Specifically against collagenases from C. histolyticum
• lgG fraction purified by affinity chromatography
• Conjugated with horseradish
• Peroxidase
• Use in ELISA and Western Blot experiments
• Immunostaining of tissue
• Analysis of residual collagenase impurities in cell preparations


* PZ activity acc. to Wϋnsch, measured at 25 ° C.


DRUG MASTER FILE FOR COLLAGENASE