Collagenase and Neutral Protease
Posted by Sara On Wed 20 Mar, 2019


Collagenases and Neutral Proteases

Collagenases are enzymes that break the peptide bonds in collagen. 

Crescent Chemical Co., Inc. supplies the following  Nordmark Collagenases and Neutral Proteases from Clostridium Histolyticum for tissue dissociation. 

Nordmark is the worlds largest producer and supplier of Collagenases.

NB 1 PremiumGrade Collagenase

NB 1 GMPGrade Collagenase

AF-1 GMPGrade Collagenase

NB 4Standard Grade Collagenase

NB 4 ProvedGrade Collagenase

NB 5 SterileGrade Collagenase

NB 6 GMPGrade Collagenase

NB 8 BroadRange Grade Collagenase

NeutralProtease NB

NeutralProtease GMP Grade

NeutralProtease AF GMP Grade

CollagenaseN Reference Content

CollagenaseN Reference Identity

AntiCollagenase

AntiCollagenase HRP



NB 1 Premium Grade
Cat. No. 17455.03
Pack Size ≥ 2,000 PZ U/Vial
  • Chromatographically highly purified
  • Class l and ll collagenases
  • Use in combination with NB Neutral Protease
  • PZ activity * ≥ 3.0 U/mg lyophilisate
  • Largely free of clostripain, trypsin-like proteases
  • Endotoxins ≥ 10 EU/mg lyophilisate
  • Collagen characterization
  • Isolation of pancreatic islets
  • Use in combination with Neutral Protease NB
  • Collagen characterization

NB 1 GMP Grade
Cat. No. 17542.01
Pack Size ≥ 2,000 PZ U/Vial
  • Enzymatic properties see NB 1 Premium Grade
  • Manufactured according to Intl. cGMP guidelines
  • Isolation of pancreatic islets for transplantation into humans
  • Use in combination with Neutral Protease NB GMP Grade

AF-1 GMP Grade
Cat. No. 17457-01
Pack Size ≥ 2,000 PZ U/Vial
  • Chromatographically highly purified
  • Class l and ll collagenases
  • Very low levels of endotoxins
  • Isolation of pancreatic islets sensitive liver, dental pulp cells

NB 4 Standard Grade
Cat. No. 17454.01/ 1 g
Cat. No. 17454.02/ 500 mg
Cat. No. 17454.03/ 5 g
  • Class l and ll collagenases and a balanced ratio of proteolytic activities
  • PZ activity* ≥ 0.1 U/mg lyophilisate
  • Isolation of various cell types including neuronal and endothelial cells, chondrocytes, adipocytes epithelial cells, stem cells, gangliocytes and periodontal cells

NB 4G Proved Grade
Cat. No. 17465.01/ 500 mg
Cat. No. 17465.02// 1 g
Cat. No. 17465.03/ 5 g
  • Class l and ll collagenases as well as a balanced mix of neutral protease, clostripain, and tryptic activities
  • PZ act.* ≥ 0.18 U/mg lyophilisate
  • Fuction tested for cell isolation
  • Isolation of adipocytes, fibroblasts hepatocytes, chondrocytes, stem cells, frog oocytes, mastocytes and tumor cells

NB 5 Sterile Grade
Cat. No. 17459.03 / 1 g
  • Enzymatic properties see NB 4
  • Sterile according to European req. For medicinal prods. (Pharm. Eur.)
  • Application fields see NB 4
  • Sterile and ready to use

NB 6 GMP Grade
Cat. No. 17458.03/ 1 g
Cat. No. 17458.04/ 100 mg
  • Enzymatic properties see NB 4
  • Aseptic production according to
  • European req. for medicinal prods.(Pharm. Eur.)
  • Manufactured according to Intl. cGMP guidelines
  • Application fields see NB 4
  • Production of cell as well as tissue engineering for transplantation into humans

NB 8 Broad Range
Cat. No. 17456.01/ 250 mg
Cat. No. 17456.02/ 1 g
  • Class l and ll collagenases and reduced levels of clostripain, trypsin-like activities and neutral protease
  • PZ act.* ≥ 0.9 U/mg lyophilisate
  • Endotoxins ≥ 100 EU/mg lyophilisate
  • Isolation of cardiomyocytes, porcine hepatocytes, rodent pancreatic islets tumor and thyroid cells

NB Neutral Protease
Cat. No. 30301.11/ 50 U (DMC)
Cat. No. 30301.12/ 100 U (DMC)
  • Chromatographically purified neutral protease.
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB

NB Neutral Protease
High Active Grade

Cat. No. 30302.02/ 80 U (DMC)
  • Chromatographically purified and contains an increased proteolytic activity
  • Largely free from collagenolytic activity (PZ U)
  • Endotoxin level is very low
  • Activity (DMC assay) ≥ 4.00 U/mg
  • Endotoxin ≥ 50.0 EU/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB 1 Premium Grade or another Collagenase NB product

NB Neutral Protease GMP Grade
Cat. No. 17452.01/ ≥ 2,000 U (PZ)
  • Chromatographically purified and largely free from collagenolytic activity (PZ U) and endotoxins
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB 1 GMP Grade or Collagenase NB 6 GMP Grade from various tissue types for clinical applications.

NB Neutral Protease AF GMP Grade
Cat No. 30306.01/ ≥ 2,000 U (PZ)
  • Chromatographically purified and largely free from collagenolytic activity (PZ U) as well as endotoxins
  • Absent of any animal-derived ingredients and thorough microbial analysis provide the highest possible safety
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase AF-1 GMP Grade from several tissue types for clinical applications

Collagenase N
Reference Content / ≥ 50 mg
Cat. No. 17463
  • Class l and ll collagenases and other proteolytic enzymes
  • Collagenase activity is regularly reevaluated
  • Reference substance for enzyme activity determination.

Collagenase N
Reference Identity / ≥ 50 mg
Cat. No. 17464
  • Class I and ll collagenases and other proteolytic enzymes
  • Reference substance for SDS-PAGE analysis, for incoming goods inspection according to cGMP

Anti-Collagenase
Cat. No. 58050 / 100 μ
  • Specifically against collagenases from C. histolyticum
  • lgG fraction purified by Protein G affinity chromatography
  • Use in ELISA and Western Blot experiments
  • Immunostaining of tissue
  • Analysis of residual collagenase impurities in cell preparations

Anti-Collagenase HRP
Cat. No. 58051 / 100 μ
  • Specifically against collagenases from C. histolyticum
  • lgG fraction purified by affinity chromatography
  • Conjugated with horseradish
  • Peroxidase
  • Use in ELISA and Western Blot experiments
  • Immunostaining of tissue
  • Analysis of residual collagenase impurities in cell preparations


* PZ activity acc. to Wϋnsch, measured at 25 ° C.
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